Restriction Enzymes
Promega Restriction Enzymes - Nde I (D)
Active and capable of digesting DNA in 15 minutes or less, directly in GoTaq™ Green Master Mix. (CA/TA TG GT AT\AC)
Promega Restriction Enzymes - Sma I Blue/White Cloning and Genome Qualified (J)
XmaI is a neoschizomer of SmaI. Available at high concentration, containing 5,000 units of SmaI at a concentration of 40-80u/μL. (CCC/GGG GGG\CCC)
Promega Restriction Enzymes - Dpn I (B)
Capable of digesting DNA in 15 minutes or less. (G(me)A/TC CT\(me)AG)
Promega Restriction Enzymes - Nco I Blue/White Cloning Qualified (D)
Capable of digesting DNA in 15 minutes or less. (C/CATG G G GTAC\C)
Promega Restriction Enzymes - EcoR V Blue/White Cloning Qualified (D)
Capable of digesting DNA in 15 minutes or less. (GAT/ATC CTA\TAG)
Promega Restriction Enzymes - Not I Blue/White Cloning and Genome Qualified (D)
Capable of digesting DNA in 15 minutes or less. (GC/GGCC GC CG CCGG\CG)
Promega Restriction Enzymes - Sgf I Genome Qualified (C)
Genome qualified to ensure optimal performance in genomic analysis applications. Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications. (GCG AT/CGC CGC\TA GCG)
Promega Restriction Enzymes - Spe I Blue/White Cloning and Genome Qualified (B)
Capable of digesting DNA in 15 minutes or less. (A/CTAG T T GATC\A)
Promega Restriction Enzymes - EcoR I Blue/White Cloning Qualified (H)
Capable of digesting DNA in 15 minutes or less. (G/AATT C C TTAA\G)
Promega Restriction Enzymes - Mlu I Blue/White Cloning and Genome Qualified (D)
Genome qualified to ensure optimal performance in genomic analysis applications. This Mlul products is active and capable of digestion directly in GoTaq™ Green Master Mix. (A/CGCG T T GCGC\A)
Dryzyme™ Restriction Enzymes
Three most frequently used restriction enzymes for biotechnology.
New England Biolabs, Inc. SpeI-HF – 500 units
A high fidelity restriction endonuclease that recognizes the sequence A^CTAGT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. Blunt/TA Ligase Master Mix – 50 reactions
Blunt/TA Ligase Master Mix is a ready-to-use solution of T4 DNA Ligase, proprietary ligation enhancer, and optimized reaction buffer. This master mix is specifically formulated to improve ligation and transformation of both blunt-end and single-base overhang substrates. The master mix format simplifies reaction set-up, ensures an optimized ratio of enzyme and buffer components, and yields robust, rapid ligation of all types of DNA ends using a short incubation time at room temperature. No thawing is necessary as it remains liquid during storage at -20C. Ligations for subcloning can be carried out in small volumes with low DNA concentrations, allowing users to conserve precious DNA samples and directly transform many strains of chemically competent E. coli without dilution.
New England Biolabs, Inc. BamHI-HF – 10000 units
A high fidelity restriction endonuclease that recognizes the sequence G^GATC_C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.