Restriction Enzymes
Promega Restriction Enzymes - Nde I (D)
Active and capable of digesting DNA in 15 minutes or less, directly in GoTaq™ Green Master Mix. (CA/TA TG GT AT\AC)
Promega Restriction Enzymes - Dpn I (B)
Capable of digesting DNA in 15 minutes or less. (G(me)A/TC CT\(me)AG)
Promega Restriction Enzymes - BamH I Blue/White Cloning Qualified (E)
Capable of digesting DNA in 15 minutes or less. Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications. (G/GATC C C CTAG\G)
Promega Restriction Enzymes - Nco I Blue/White Cloning Qualified (D)
Capable of digesting DNA in 15 minutes or less. (C/CATG G G GTAC\C)
Promega Restriction Enzymes - Not I Blue/White Cloning and Genome Qualified (D)
Capable of digesting DNA in 15 minutes or less. (GC/GGCC GC CG CCGG\CG)
Promega Restriction Enzymes - EcoR V Blue/White Cloning Qualified (D)
Capable of digesting DNA in 15 minutes or less. (GAT/ATC CTA\TAG)
Promega Restriction Enzymes - Sgf I Genome Qualified (C)
Genome qualified to ensure optimal performance in genomic analysis applications. Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications. (GCG AT/CGC CGC\TA GCG)
Promega Restriction Enzymes - Mlu I Blue/White Cloning and Genome Qualified (D)
Genome qualified to ensure optimal performance in genomic analysis applications. This Mlul products is active and capable of digestion directly in GoTaq™ Green Master Mix. (A/CGCG T T GCGC\A)
Promega Restriction Enzymes - Spe I Blue/White Cloning and Genome Qualified (B)
Capable of digesting DNA in 15 minutes or less. (A/CTAG T T GATC\A)
Promega Restriction Enzymes - EcoR I Blue/White Cloning Qualified (H)
Capable of digesting DNA in 15 minutes or less. (G/AATT C C TTAA\G)
Dryzyme™ Restriction Enzymes
Three most frequently used restriction enzymes for biotechnology.
New England Biolabs, Inc. EcoRI-HF – 10000 units
A high fidelity restriction endonuclease that recognizes the sequence G^AATT_C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. SpeI-HF – 500 units
A high fidelity restriction endonuclease that recognizes the sequence A^CTAGT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. BamHI-HF – 10000 units
A high fidelity restriction endonuclease that recognizes the sequence G^GATC_C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.